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Welcome

Welcome to the Structural Biology Facility. Since its inception in 2004, the Structural Biology Facility has trained research technicians, and established protein expression, purification and crystallization laboratories accessible to all.

Purpose

The overall purpose of the Structural Biology Facility is to provide access to state of the art structural biology instrumentation for the purpose of applying structural techniques to the analysis of important cancer-related biological macromolecules, providing basic knowledge of disease mechanism, driving research and directing the synthesis of novel therapeutics.

Atomic images of the arrangement of amino acid side chains in three dimensions gives the details needed to visualize the active sites of enzymes, see the DNA binding sites of transcription factors and view the protein-protein interactions of signaling molecules. Function can be understood through determination of atomic structures. Modification of the function of macromolecules is a key to developing specific therapies without side effects.

Facilities

The Structural Biology Facility is divided into four main laboratory components:

  1. Protein Expression and Purification (PrEP) Lab - This lab is dedicated to the high yield production, purification and labeling of protein.
  2. Crystal Screening and Growth (CSG) Lab - This lab uses the Formulatrix industry-standard automated crystallization platform to set up, image and electronically evaluate experiments.
  3. X-ray Crystallography Lab - This lab has a Rigaku protein crystallography instrument composed of an FRE rotating anode generator with an R-Axis IV++ detector configured for the collection of high resolution X-ray diffraction data on one port and a Rigaku BioSAXS-1000 on the other port.

Access

Access to the Structural Biology Facility is arranged through Dr. Borgstahl.  Contact her through the web here or by phone at 9-8578.

Funding

The Structural Biology Facility is supported in part by the following...

univ_neb_nri.jpg Nebraska Research Initiative

neepscorlogo.gifNebraska EPSCoR IDeA Program

nihlogo.gif National Institutes of Health

nsflogo.gif National Science Foundation

eppleylogo-esbf.jpg 

 

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