- Info
Fees - 2009 to 2010
The page contains the fee structure used by the Eppley Structural Biology Facility for 2009-2010.
|
ECC
|
Other
|
Optimizing Protein Expression
|
|
|
|
|
free |
free |
|
|
$150 |
$225 |
- Special cases: Artic Express RIL host, etc. (price per each condition)
|
$100 |
$135 |
|
|
$70 |
$100 |
- Plasmid prep/ backup storage of plasmid
|
$30 |
$50 |
| |
|
|
Protein Purification
|
|
|
- Develop 2-step protocol (2 dedicated different columns, buffers, optimization trials)
|
$250 |
$335 |
- Tag removal (up to 10 mg of protein)
|
|
|
| Thrombin |
$20 |
$30 |
| HRV-3C |
$55 |
$75 |
| Factor Xa |
$95 |
$125 |
- Extra steps or specialized purification (gel filtration, MonoQ, MonoS, etc.)
|
$100 |
$135 |
- Extra gel analyses (per minigel)
|
$15 |
$20 |
- Limited proteolysis analysis (assay, mass spectroscopy, cloning recommendations)
|
Contact |
Contact |
- Dynamic Light Scattering (for monodispersity)
|
Contact |
Contact |
- SecMals analysis (monodispersity, aggregation, molecular wt of product)
|
Contact |
Contact |
| |
|
|
Production of Quantity of Purified Protein
|
|
|
- Production via known protocol, cost per 4 liters of culture
Protein purity suitable for crystallization experiments
|
$60 |
$80 |
- Labeled protein for NMR (15N, 13C, D2O), per 4 liters of culture
Estimated from $200 for 15N to $500 for 13C
|
Contact |
Contact |
- Large quantities produced from Fermentor (15L batch run)
|
$325 |
$450 |
| |
|
|
Crystallization Services
|
|
|
|
Services are available for proteins purified in our labs or in your own. Provided documentation of purity for samples from other labs will be required. Details of those requirements are available on request.
|
|
|
- First screening: 3 commercial screens, 96 conditions each
Includes Nextel Classics, PEGS, and Anions
Requires at least 150μL of protein at 8-10 mg/mL concentration
Includes 2 months of automated monitoring with RockMaker
|
$125 |
$170 |
- Additional screens, 96 conditions each, as requested
|
$50 |
$70 |
- Optimization of crystals from screening hits, per 24 or 96 well screen, by hand or robot-mediated
|
$20 |
$30 |
| |
|
|
X-Ray Diffraction of Crystal for Structural Data
|
|
|
- Screening: Technician will do trial diffractions on early crystals (cost per hour logged)
|
$30 |
$40 |
- Data acquisition: charge per day of beam time
|
$100 |
$150 |
| |
|
|
NMR Data Acquisition
|
|
|
- Total cost is highly variable depending on type of experiment that is run (Charge per hour)
|
$10 |
$10 |
| |
|
|
Data Processing
|
|
|
- Processing and Solving of X-Ray Diffraction Data Structure
|
Contact |
Contact |
- Processing and Solving of NMR Data
|
Contact |
Contact |
