There are two tiers of experiments: (Tier 1) preliminary “Go or No Go” experiments; and (Tier 2) mature projects ready for preparing samples for the X-ray and NMR facility. Tier 1 experiments are the riskiest with no guarantee of success so fees for Tier 1 experiments are based on supply costs only. For Tier 2 fees, these experiments require a lot of time, so staff time is included along with the supply costs. Limited personal use of some of the equipment may be arranged by permission. (fees shown at the bottom) Once the X-ray or NMR data are collected, the processing of these data and subsequent structure solution are not provided by ESBF staff. Collaboration with one of the structural biology faculty is recommended.
TIER 1 Fees1: “Go or No Go” experiments are negotiated and performed in a collaborative fashion and fees are based on supply costs only. |
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Optimizing Protein Expression |
UNMC – Related | Other |
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free | free |
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$215 | $325 |
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$125 | $190 |
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$160 | $240 |
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$40 | $60 |
Protein Purification |
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$325 | $490 |
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Thrombin | $25 | $40 |
Precision protease, HRV-3C | $130 | $195 |
Factor Xa | $150 | $225 |
Enterokinase | $400 | $600 |
AcTEV protease | $500 | $750 |
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Size exclusion, MonoQ, other columns | $100 | $135 |
Extra gel analyses (per minigel) | $25 | $40 |
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Contact | Contact |
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$30 | $45 |
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$25/hr | $40/hr |
TIER 2 Fees1: Experiments are done upon request and supply-based fees plus staff time at $30/hr are charged. The request form is available on line and the completed form should be emailed to Carol Kolar. The form can be edited and the changes can be saved with acrobat reader. | ||
Production of Large Quantities of Purified Protein |
UNMC – Related | Other |
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$100 | $150 |
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$30 | $45 |
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$250 | $375 |
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$400 | $600 |
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Catalog $ | Catalog $ |
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Contact | Contact |
Crystallization Services |
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Services are available for proteins purified in our labs or in your own. Documentation of purity for samples from other labs will be required. Details of those requirements are available on request. | ||
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$160 | $240 |
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$50 | $75 |
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$65 | $100 |
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$50 | $75 |
X-Ray Diffraction of Crystal for Structural Data |
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$6 | $9 |
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$125 | $200 |
NMR Services |
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Current Fees for NMR can be found HERE. | ||
Personal Use: Individuals who would like to work with equipment in the facility on their own.Note: All usage is subject to added charges for technical time as needed for training, advising and monitoring equipment at $30/hour. Effective: October 1, 2011 |
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Equipment available for personal use with permission |
UNMC – Related | Other |
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$25 | NA |
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$25 | NA |
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$25 | NA |
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$50 | NA |
1Prices are subject to change with a 30 day notice.