There are two tiers of experiments: (Tier 1) preliminary “Go or No Go” experiments; and (Tier 2) mature projects ready for preparing samples for the X-ray and NMR facility. Tier 1 experiments are the riskiest with no guarantee of success so fees for Tier 1 experiments are based on supply costs only. For Tier 2 fees, these experiments require a lot of time, so staff time is included along with the supply costs. Limited personal use of some of the equipment may be arranged by permission. (fees shown at the bottom) Once the X-ray or NMR data are collected, the processing of these data and subsequent structure solution are not provided by ESBF staff. Collaboration with one of the structural biology faculty is recommended.
TIER 1 Fees
TIER 1 Fees1: “Go or No Go” experiments are negotiated and performed in a collaborative fashion and fees are based on supply costs only.
| UNMC | Other | |||
|---|---|---|---|---|
| Optimizing Protein Expression | ||||
| Initial consultation and evaluation of sequence | free | free | ||
| Standard Protocol (2 cell hosts, 2 temperature conditions, IPTG optimized, gels) | $215 | $325 | ||
| Special cases: Artic Express RIL host, C19, C41, C43 etc. (price per trial) | $125 | $190 | ||
| Insect cell host (Sf9; 250ml culture, protein expression, w. blot) | $160 | $240 | ||
| Plasmid prep/ backup storage of plasmid | $40 | $60 | ||
| Protein Purification | ||||
| Develop 2-step protocol (2 dedicated different columns, buffers, optimization trials) | $325 | $495 | ||
| Tag removal (up to 10 mg of protein) | ||||
| Thrombin | $25 | $40 | ||
| Precision protease, HRV-3C | $130 | $195 | ||
| Factor Xa | $150 | $225 | ||
| Enterokinase | $400 | $600 | ||
| AcTEV protease | $500 | $750 | ||
| Extra steps or specialized purification | ||||
| Size exclusion, MonoQ, other columns | $100 | $135 |
||
| Extra gel analyses (per minigel) | $25 | $40 | ||
| Limited proteolysis analysis (assay, mass spectroscopy, cloning recommendations) | contact | contact | ||
| Sample concentration | $30 | $45 | ||
| $25/hr | $40/hr | |||
TIER 2 Fees
TIER 2 Fees1: Experiments are done upon request and supply-based fees plus staff time at $30/hr are charged. The request form is available on line and the completed form should be emailed to Lucas Struble. The form can be edited and the changes can be saved with acrobat reader.
| UNMC | Other | |||
|---|---|---|---|---|
| Production of Large Quantities of Purified Protein | ||||
| For Crystallization Experiments: Production via known protocol, cost per 4 liters of culture | $100 | $150 | ||
| Sample concentration | $30 | $45 | ||
| Large batch purification (15-25ml resin) | $250 | $375 | ||
| Large quantities produced from Fermentor (15L batch run) | $400 | $600 | ||
| New Columns as Necessary | Catalog $ | Catalog $ | ||
| Labeled protein for NMR (15N, 13C, D2O), per 4 liters of culture Estimated from $250 for 15N to $625 for 13C | Contact | Contact | ||
| Crystallization Services | ||||
| Services are available for proteins purified in our labs or in your own. Documentation of purity for samples from other labs will be required. Details of those requirements are available on request. | ||||
| First screening: 3 commercial screens, 96 conditions each Includes NeXtal Classics, PEGS, and Anions Requires at least 350μL of protein at 8-10 mg/ml concentration Includes 2 months of automated monitoring with RockMaker | $160 | $240 | ||
| Complex formation (DNA or protein binding partner) | $50 | $75 | ||
| Additional screens, 96 conditions each, as requested | $65 | $100 | ||
| Optimization of crystals from screening hits, per 24 or 96 well screen, by hand or robot-mediated | $50 | $75 | ||
| X-Ray Diffraction of Crystal for Structural Data | ||||
| Screening: Technician will do trial diffractions on early crystals (cost per crystal mounted) | $6 | $9 | ||
| Data acquisition: charge per 24 hours of beam time | $125 | $200 |
||
| NMR Services | ||||
| Current Fees for NMR can be found HERE. | ||||
Personal Use: Individuals who would like to work with equipment in the facility on their own.
Note: All usage is subject to added charges for technical time as needed for training, advising and monitoring equipment at $30/hour.
Effective: October 1, 2011
| UNMC | Other | |||
|---|---|---|---|---|
| Equipment available for personal use with permission | ||||
| Tecan Robot, per hour of use | $25 | na | ||
| Emulsiflex C3, per lysis | $25 | na | ||
| New Brunswick Shaker/Incubator, per use | $25 | na | ||
| AKTA FPLC, AKTA Explorer, per purification day | $50 | na | ||
1Prices are subject to change with a 30 day notice.